Studies on Sedative Effect of
Relazovac®
Abstract:
6g, 3g, and
1.5g/kg (weight) of Relazovac®,
equivalent to 40g, 20g, and 10g of crude drugs, which was respectively 17, 8.5,
and 4 times of the dosage for adults, were fed (ig) to mice for 14 days. As a
result, the number of mice that fell asleep was increased in the sub-threshold
dose of pentobarbital sodium (30mg/kg). The percentage of sleeping is 42.86,
35.71 and 7.14% respectively while that of the control group was zero. The
sleeping span of mice in the threshold dose of sodium amytal was prolonged and
the incubation period was shortened. It is proven that the medicine has the
inhibitory effect on the central nervous system.
Materials:
1) Relazovac® extract was provided by LIFEnhance, Inc. with
lot number 20020403. 0.15g extract was
equivalent to 1 g crude drugs. As required by the experiment, the extract was
dissolved in distilled water to various concentrations and stored in the refrigerator
at 4C until use.
2) Pentobarbital sodium was provided by
Shanghai Branch of Chemical Reagent Packing, with batch number 940612.
3) Sodium
amytal was provided by Shanghain Supplying Station of Chemical Reagent of the
Medicine Company of
4)
Estazolam was provided by AiBaike Medicine Industry, with batch number
20010402.
5) Diazepam
was provided by Shanghai Jiu Fu Medicine Industry, with batch number 000201.
The Animals,
provided by
Methods
and Results:
1.
The effect of Relazovac® on the sub-threshold dose of pentobarbital
sodium.
According
to the literature [1], the mice, with the weight of 18-22g and the
same number of ♀♂, were divided into five groups at random, each
with 14 mice: the control group, Diazepam positive group and groups of Relazovac® with large,
medium, and small dosages. The mice were respectively given (i.g) NS 20ml/kg,
0.5mg of diazepam/kg, 6g, 3g and 1.5g of Relazovac®/kg
weight (equivalent to 40g, 20g and 10g of crude drugs, and 17.09, 8.57 and 4.27
times of the dosage for adults respectively). The groups were given the
medicine (i.g) once a day on 12 consecutive days. An hour after the last dosage
of medicine, pentobarbital sodium (30mg/kg) was injected into the abdominal
cavity of the mice. The righting reflex of the mice was faded for more than one
minute, which was taken as the sleeping index to calculate the sleeping
percentage of the mice. The statistics was as follows (see Table1)
Table1.
The effect of Relazovac®
on the sub-threshold dose of pentobarbital sodium
|
Groups |
The number of mice |
Dosage (/kg) |
Percentage of sleeping |
P-value |
|
The
control group |
14 |
Ns20ml |
0 |
0 |
|
Diazepam
group |
14 |
0.5mg |
64.29 |
<0.01 |
|
Large
dosage group |
14 |
6g |
42.86 |
<0.05 |
|
Medium
dosage group |
14 |
3g |
35.71 |
<0.05 |
|
Small
dosage group |
14 |
1.5g |
7.14 |
>0.05 |
Table 1
shows the sleeping percentage of mice is increased in the sub-threshold dose of
Pentobarbital sodium. It is proven that the medicine Relazovac ®
has the inhibitory effect on the central nervous system.
2. The effect of Relazovac ®
on the mice’s sleeping time of sodium amytal.
According
to the literature[2], the mice, half of whom were male, with the
weight of 18-22g were divided into groups at random, each with 12 mice, that
is, the control group, Estazolam group and groups of Relazovac® with large, medium and small dosages. They
were respectively given (i.g) once a day NS 20ml/kg, 0.5mg/kg of Estazolam, and
6g, 3g and 1.5g/kg of Relazovac® continuously for 14 days. An
hour after the last dosage of medicine, sodium amytal (50mg/kg) was injected
into the abdominal cavity of the mice. The time of falling asleep was from the
fade of the righting reflex and the time from the fade of the righting reflex
to waking was the sleep span. The differences among the groups are shown in
Table 2.
Table2.
The effect of Relazovac ®
on the mice’s sleeping of sodium amytal
|
Groups |
The number |
Dosage (/kg) |
The time of falling asleep 又±SD (min) |
Sleeping span 又+SD (min) |
|
The
control |
12 |
— |
7.08±3.40 |
20.67±7.35 |
|
Estazolam |
12 |
0.5mg |
4.00±0.95** |
42.83±16.78 |
|
Large
dosage |
12 |
6g |
4.17±1.99* |
31.67±15.04* |
|
Medium
dosage |
12 |
3g |
3.83±1.03** |
31.42±11.55* |
|
Small
dosage |
12 |
1.5g |
4.42±1.16* |
30.42±14.9 |
n=12 compared with the control group
* P<0.05 , ** P<0.01, ***P<0.001
The results
show that the medicine can prolong the sleeping span in the threshold dose of
sodium amytal and the incubation period is shortened. It is proven that the
medicine Relazovac ® has
the inhibitory effect on the central nervous system.
References
[1] Chen
QI. Research Methodology of Pharmacology of Traditional Chinese Medicine〔M〕, People’s Heath Press P.672.
[2] Li
yikui. Experimental Methodology of Pharmacology of Traditional Chinese Medicine〔M〕Shanghai: Shanghai Science and
Technology Press,1991: 335.